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Biological characteristics and genomic analysis of GXEC-STL2. A Colony morphology on Columbia blood agar. Scale bar, 1 cm. B Colony morphology on EMD agar. C Gram-stained. Scale bar, 20 μm. D Transmission electron micrograph. Scale bar, 1 μm. E Comparative circular genome map. CDS, positive and negative GC, and resistance gene are indicated by blue, green, purple, and red, respectively. F Neighboring evolutionary tree between GXEC-STL2 (highlight) and 25 different genera of bacteria. The phylogenetic tree was constructed on the basis of the sequences of bacterial <t>16S</t> <t>rRNA.</t>
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Biological characteristics and genomic analysis of GXEC-STL2. A Colony morphology on Columbia blood agar. Scale bar, 1 cm. B Colony morphology on EMD agar. C Gram-stained. Scale bar, 20 μm. D Transmission electron micrograph. Scale bar, 1 μm. E Comparative circular genome map. CDS, positive and negative GC, and resistance gene are indicated by blue, green, purple, and red, respectively. F Neighboring evolutionary tree between GXEC-STL2 (highlight) and 25 different genera of bacteria. The phylogenetic tree was constructed on the basis of the sequences of bacterial 16S rRNA.

Journal: Veterinary Research

Article Title: Targeting STEC-induced edema disease in weaned piglets: prophylactic oral phage P-GXEC-L2P5 attenuates bacterial colonization, toxin production, and endothelial damage

doi: 10.1186/s13567-025-01683-w

Figure Lengend Snippet: Biological characteristics and genomic analysis of GXEC-STL2. A Colony morphology on Columbia blood agar. Scale bar, 1 cm. B Colony morphology on EMD agar. C Gram-stained. Scale bar, 20 μm. D Transmission electron micrograph. Scale bar, 1 μm. E Comparative circular genome map. CDS, positive and negative GC, and resistance gene are indicated by blue, green, purple, and red, respectively. F Neighboring evolutionary tree between GXEC-STL2 (highlight) and 25 different genera of bacteria. The phylogenetic tree was constructed on the basis of the sequences of bacterial 16S rRNA.

Article Snippet: Jejunal content samples were submitted to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S ribosomal RNA (rRNA) gene amplicon sequencing; subsequent bioinformatics analysis was performed on the QIIME2 platform.

Techniques: Staining, Transmission Assay, Bacteria, Construct